Catalog Number - 40031-SP100

40031-SP100 Cogent UDA™ (weak cation exchange, WCX) Semi‑Prep HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a uniquely powerful mixed‑mode separation mechanism that combines reversed phase, weak cation exchange, and HILIC interactions in a single stationary phase.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple retention mechanisms:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar retention capability)

This multi‑mode selectivity makes the column especially effective for semi‑preparative purification of polar, ionizable, and structurally similar compounds, particularly in complex matrices where standard prep columns fall short.

The TYPE‑C silica base provides key performance advantages:

• Faster equilibration between runs

• Reduced sensitivity to mobile phase moisture

• Robust column lifetimes for repeated purification cycles

• Minimal salt requirements in HILIC mode (MS‑friendly fractions)

100 mm Length – Balanced Resolution for Semi‑Prep Purification

The 100 mm length provides an optimal balance between throughput and resolving power for semi‑preparative workflows.

• Higher resolution than 50 mm semi‑prep columns

• Faster cycle times than longer preparative formats

• Increased peak capacity for moderately complex mixtures

✅ Recommended for routine purification, scale‑up, and method transfer from analytical columns

Flow Rate & System Requirements (10 mm ID, 4 µm)

⚠️ Requires semi‑preparative HPLC system capability

• Typical flow rate range: ~4 – 12 mL/min

• Moderate backpressure for 10 mm ID format

• Designed for higher flow purification workflows

• Compatible with fraction collectors and preparative detectors

✅ Important: A specialized HPLC system is required for columns of this size

✅ Best Practice: Use appropriate sample preparation and a matched guard column to extend column life and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Difficult Separations

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Tunable Retention

• WCX mechanism active at pH ≥ 6

• Selectivity controlled via pH and ionic strength

✅ Ideal when traditional prep C18 or ion exchange columns cannot achieve desired purity

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces cycle time and increases throughput

No Strict Moisture Control (HILIC)

• Simplifies solvent preparation

Robust Column Lifetimes

• Designed for repeated semi‑prep use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and improved downstream compatibility

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• RP, HILIC, and WCX in one column

Semi‑Prep Scale Capability (10 mm ID)

• Supports purification and scale‑up applications

Balanced Performance (4 µm Particles)

• Efficient separation with manageable backpressure

Typical Applications

• Semi‑preparative HPLC purification

• Analytical to prep scale‑up

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires semi‑preparative HPLC system capability

• Designed for high‑flow operation (10 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.